A quadrivalent norovirus vaccine based on a chimpanzee adenovirus vector induces potent immunity in mice.
Virol Sin
; 39(4): 675-684, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-38997087
ABSTRACT
Norovirus (NoV) infection is a major cause of gastroenteritis worldwide. The virus poses great challenges in developing vaccines with broad immune protection due to its genetic and antigenic diversity. To date, there are no approved NoV vaccines for clinical use. Here, we aimed to develop a broad-acting quadrivalent NoV vaccine based on a chimpanzee adenovirus vector, AdC68, carrying the major capsid protein (VP1) of noroviral GI and GII genotypes. Compared to intramuscular (i.m.), intranasal (i.n.), or other prime-boost immunization regimens (i.m. â+ âi.m., i.m. â+ âi.n., i.n. â+ âi.m.), AdC68-GI.1-GII.3 (E1)-GII.4-GII.17 (E3), administered via i.n. â+ âi.n. induced higher titers of serum IgG antibodies and higher IgA antibodies in bronchoalveolar lavage fluid (BALF) and saliva against the four homologous VP1s in mice. It also significantly stimulated the production of blocking antibodies against the four genotypes. In response to re-stimulation with virus-like particles (VLP)-GI.1, VLP-GII.3, VLP-GII.4, and VLP-GII.17, the quadrivalent vaccine administered according to the i.n. â+ âi.n. regimen effectively triggered specific cell-mediated immune responses, primarily characterized by IFN-γ secretion. Furthermore, the preparation of this novel quadrivalent NoV vaccine requires only a single recombinant adenovirus to provide broad preventive immunity against the major GI/GII epidemic strains, making it a promising vaccine candidate for further development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Vaccines
/
Adenoviridae
/
Pan troglodytes
/
Caliciviridae Infections
/
Norovirus
/
Genetic Vectors
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Mice, Inbred BALB C
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Antibodies, Viral
Limits:
Animals
Language:
En
Journal:
Virol Sin
Journal subject:
VIROLOGIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Países Bajos