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Physicochemical and Rheological Properties of Degraded Konjac Gum by Abalone (Haliotis discus hannai) Viscera Enzyme.
Lin, Zhao-Ming; Wen, Jia-Xin; Lin, Duan-Quan; Liu, Kang; Chen, Yu-Lei; Miao, Song; Cao, Min-Jie; Sun, Le-Chang.
Affiliation
  • Lin ZM; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
  • Wen JX; National & Local Joint Engineering Research Center of Deep Processing Technology for Aquatic Products, Jimei University, Xiamen 361021, China.
  • Lin DQ; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
  • Liu K; National & Local Joint Engineering Research Center of Deep Processing Technology for Aquatic Products, Jimei University, Xiamen 361021, China.
  • Chen YL; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
  • Miao S; National & Local Joint Engineering Research Center of Deep Processing Technology for Aquatic Products, Jimei University, Xiamen 361021, China.
  • Cao MJ; Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China.
  • Sun LC; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Foods ; 13(13)2024 Jul 08.
Article in En | MEDLINE | ID: mdl-38998663
ABSTRACT
In the present study, a new degraded konjac glucomannan (DKGM) was prepared using a crude enzyme from abalone (Haliotis discus hannai) viscera, and its physicochemical properties were investigated. After enzymatic hydrolysis, the viscosity of KGM obviously decreased from 15,500 mPa·s to 398 mPa·s. The rheological properties analysis of KGM and DKGMs revealed that they were pseudoplastic fluids, and pseudoplasticity, viscoelasticity, melting temperature, and gelling temperature significantly decreased after enzymatic hydrolysis, especially for KGM-180 and KGM-240. In addition, the molecular weight of KGM decreased from 1.80 × 106 Da, to 0.45 × 106 Da and the polydispersity index increased from 1.17 to 1.83 after 240 min of degradation time. Compared with natural KGM, the smaller particle size distribution of DKGM further suggests enzyme hydrolysis reduces the aggregation of molecular chains with low molecular weight. FT-IR and FESEM analyses showed that the fragmented KMG chain did not affect the structural characteristics of molecular monomers; however, the dense three-dimensional network microstructure formed by intermolecular interaction changed to fragment microstructure after enzyme hydrolysis. These results revealed that the viscosity and rheological properties of KGM could be controlled and effectively changed using crude enzymes from abalone viscera. This work provides theoretical guidance for the promising application of DKGM in the food industry.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Foods Year: 2024 Document type: Article Affiliation country: China Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Foods Year: 2024 Document type: Article Affiliation country: China Country of publication: Suiza