[Damage and changes in expression of autophagy related factors LC3 and p62 of condylar cartilage in fluorosis mouse].
Shanghai Kou Qiang Yi Xue
; 33(2): 113-116, 2024 Apr.
Article
in Zh
| MEDLINE
| ID: mdl-39005084
ABSTRACT
PURPOSE:
To study the damage and the expression of LC3 and p62 of condylar cartilage in fluorosis mouse.METHODS:
Thirty 4-week-old male C57BL/6 mice were randomly divided into control group and the experimental group with 15 animals in each group. The control group received regular drinking water and the experimental group received a fluoride concentration of 75 mg/L drinking water for 8 weeks. The structure of condylar cartilage was observed through modified safranine O-fast green FCF cartilage stain kit. Immunohistochemistry was used to detect the expression of MMP-13, type â ¡ collagen and LC3 and p62. Two-way analysis of variance test was conducted for analysis of semi-quantitative results of immunohistochemistry using SPSS 22.0 software package.RESULTS:
Compared with the control group, the fibrocartilage layer of the experimental group became thinner, the condrocytes were smaller, and the staining became deeper.Immunohistochemistry results showed that the expression of MMP-13 and LC3 increased; the expression of type â ¡ collagen and p62 decreased in the experimental group.CONCLUSIONS:
There was degeneration of the condylar cartilage and autophagy in mice with drinking water containing 75 mg/L fluoride.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autophagy
/
Matrix Metalloproteinase 13
/
Mice, Inbred C57BL
/
Microtubule-Associated Proteins
/
Fluorosis, Dental
Limits:
Animals
Language:
Zh
Journal:
Shanghai Kou Qiang Yi Xue
Journal subject:
ODONTOLOGIA
Year:
2024
Document type:
Article
Country of publication:
China