The effect of centromere protein Fta2 phosphorylation during meiosis.
Yi Chuan
; 46(7): 552-559, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-39016088
ABSTRACT
During meiosis, defects in cohesin localization within the centromere region can result in various diseases. Accurate cohesin localization depends on the Mis4-Ssl3 loading complex. Although it is known that cohesin completes the loading process with the help of the loading complex, the mechanisms underlying its localization in the centromere region remain unclear. Previous studies suggest cohesin localization in the centromere is mediated by phosphorylation of centromeric proteins. In this study, we focused on the Fta2 protein, a component of the Sim4 centromere protein complex. Using bioinformatics methods, potential phosphorylation sites were identified, and fta2-9A and fta2-9D mutants were constructed in Schizosaccharomyces pombe. The phenotypes of these mutants were characterized through testing thiabendazole (TBZ) sensitivity and fluorescent microscopy localization. Results indicated that Fta2 phosphorylation did not impact mitosis but affected chromosome segregation during meiosis. This study suggests that Fta2 phosphorylation is vital for meiosis and may be related to the specific localization of cohesin during this process.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schizosaccharomyces
/
Schizosaccharomyces pombe Proteins
/
Meiosis
Language:
En
Journal:
Yi Chuan
Journal subject:
GENETICA
Year:
2024
Document type:
Article
Affiliation country:
China