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Epigenomic Profiling of B Cell Subsets by CUT&Tag.
Akdogan-Ozdilek, Bagdeser; George-Alexander, Lou-Ella M M; Scharer, Christopher D.
Affiliation
  • Akdogan-Ozdilek B; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
  • George-Alexander LMM; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
  • Scharer CD; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA. cdschar@emory.edu.
Methods Mol Biol ; 2826: 65-77, 2024.
Article in En | MEDLINE | ID: mdl-39017886
ABSTRACT
Epigenetic programs play a key role in regulating the development and function of immune cells. However, conventional methods for profiling epigenetic mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identifying histone modifications in human and mouse lymphocytes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Histones / B-Lymphocyte Subsets / Epigenesis, Genetic / Epigenomics Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Histones / B-Lymphocyte Subsets / Epigenesis, Genetic / Epigenomics Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos