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Conversion of Mouse-Derived Hybridomas to Tasmanian Devil Recombinant IgG Antibodies.
Slyp, Bailey; Darby, Jocelyn M; Flies, Andrew S.
Affiliation
  • Slyp B; Menzies Institute for Medical Research, College of Health and Medicine, University of Tasmania, Hobart, TAS, Australia.
  • Darby JM; Menzies Institute for Medical Research, College of Health and Medicine, University of Tasmania, Hobart, TAS, Australia.
  • Flies AS; Menzies Institute for Medical Research, College of Health and Medicine, University of Tasmania, Hobart, TAS, Australia. Andy.Flies@utas.edu.au.
Methods Mol Biol ; 2826: 231-249, 2024.
Article in En | MEDLINE | ID: mdl-39017897
ABSTRACT
The hybridoma method for production of monoclonal antibodies has been a cornerstone of biomedical research for several decades. Here we convert the monoclonal antibody sequence from mouse-derived hybridomas into a "devilized" recombinant antibody with devil IgG heavy chain and IgK light chain. The chimeric recombinant antibody can be used in functional assays, immunotherapy, and to improve understanding of antibodies and Fc receptors in Tasmanian devils. The process can be readily modified for other species.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin G / Hybridomas / Marsupialia Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Australia Country of publication: Estados Unidos
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin G / Hybridomas / Marsupialia Limits: Animals Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Australia Country of publication: Estados Unidos