Different activation of STAT1 and STAT2 phosphorylation by IFNc, IFNd, and IFNh in tilapia.
Fish Shellfish Immunol
; 152: 109776, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-39019128
ABSTRACT
Type I IFNs are a subset of cytokines exerting their antiviral effects mainly through the JAK-STAT signalling. Immunogenetic studies have shown that fish possess key components of IFN-JAK-STAT cascade, but the information about the distinct responses of STAT1 and STAT2 to different IFNs is rather limited in fish. Here, we identified and cloned STAT1 and STAT2 genes (named as On-STAT1 and On-STAT2) from tilapia, Oreochromis niloticus. On-STAT1 and On-STAT2 genes were detected in all orangs/tissues examined, and were rapidly induced in spleen, head kidney, and liver following the stimulation of poly(IC). In addition, the stimulation of poly(IC), poly(AT), and different subgroups of recombinant IFNs could induce the expression of On-STAT1 and On-STAT2 in TA-02 cells with distinct induction levels. Importantly, On-STAT2 was rapidly phosphorylated by all three subgroups of IFNs, but the phosphorylation of On-STAT1 was only observed in IFNc- and IFNh-treated TA-02 cells, reflecting the distinct activation of STAT by different subgroups of fish IFNs. The present results thus contribute to better understanding of the JAK-STAT signalling mediated by different subgroups of IFNs in fish.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fish Proteins
/
STAT1 Transcription Factor
/
STAT2 Transcription Factor
Limits:
Animals
Language:
En
Journal:
Fish & shellfish immunology (Online)
/
Fish Shellfish Immunol
/
Fish shellfish immunol
Journal subject:
BIOLOGIA
/
MEDICINA VETERINARIA
Year:
2024
Document type:
Article
Country of publication:
Reino Unido