Microbial upcycling of methane to phytoene using metabolically engineered Methylocystis sp. MJC1 strain.
Bioresour Technol
; 407: 131116, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-39019197
ABSTRACT
Methane, a potent greenhouse gas, requires sustainable mitigation strategies. Here, the microbial upcycling of methane to phytoene, a valuable colorless carotenoid with applications in the cosmeceutical industry was demonstrated. To achieve this goal, a stepwise metabolic engineering approach was employed in Methylocystis sp. MJC1, a methane-oxidizing bacterium. The incorporation of crtE and crtB genes from Deinococcus radiodurans R1 established the phytoene biosynthetic pathway. This pathway was fine-tuned through promoter optimization, resulting in a phytoene production of 450 µg/L from 37 mmol/L methane. Disrupting the ackA gene reduced a by-product, acetate, by 50 % and increased phytoene production by 56 %. Furthermore, overexpressing the dxs gene boosted phytoene titer 3-fold. The optimized strain produced 15 mg/L phytoene from 2 mol/L methane in fed-batch fermentation, a 4-fold increase in phytoene titer and 4-fold in yield. This demonstrates Methylocystis sp. MJC1's potential for efficient phytoene production and presents a novel approach for greenhouse gas reduction.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Methylocystaceae
/
Metabolic Engineering
/
Methane
Language:
En
Journal:
Bioresour Technol
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2024
Document type:
Article
Country of publication:
Reino Unido