Development and application of a multi-sugar assay to assess intestinal permeability.
Bioanalysis
; 16(16): 849-861, 2024.
Article
in En
| MEDLINE
| ID: mdl-39023344
ABSTRACT
Aim:
Bioanalytical assays to measure rhamnose, erythritol, lactulose and sucralose in human urine and plasma were developed to support an indomethacin challenge study for intestinal permeability assessment in healthy participants.Methods:
The multi-sugar assays utilized 5-µl sample matrix and a simple chemical derivatization with acetic anhydride, followed by RPLC-MS/MS detection.Results:
Rhamnose and erythritol quantification was established between 1.00-1,000 µg/ml in urine and 250-250,000 ng/ml in plasma. For lactulose and sucralose, dynamic ranges of 0.1-100 µg/ml (urine) and 25-25,000 ng/ml (plasma) were applied for biological measurements.Conclusion:
This work helped overcome some of the common analytical challenges associated with the bioanalysis of mono- and disaccharides and achieved improved limits of quantification.
[Box see text].
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Permeability
/
Tandem Mass Spectrometry
Limits:
Humans
Language:
En
Journal:
Bioanal. (Online)
/
Bioanalysis
/
Bioanalysis (Online)
Year:
2024
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Reino Unido