Protocol to evaluate rat and mouse cardiomyocyte proliferation in vitro and in vivo.
STAR Protoc
; 5(3): 103204, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-39037896
ABSTRACT
Here, we present a protocol for the quantitative assessment of rat and mouse cardiomyocyte proliferation both in vitro and in vivo. For the in vivo approach, we describe steps for the isolation of neonatal rat cardiomyocytes and the employment of various indicators to quantify cell proliferation. We then detail in vivo procedures that incorporate comprehensive assays and a genetic lineage tracing strategy to evaluate endogenous cardiomyocyte proliferation. This protocol can be modified to investigate other mammalian cardiomyocyte proliferation. For complete details on the use and execution of this protocol, please refer to Ji et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Myocytes, Cardiac
/
Cell Proliferation
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Estados Unidos