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Markers of Dermal Fibroblast Subpopulations for Viable Cell Isolation via Cell Sorting: A Comprehensive Review.
Luszczynski, Krzysztof; Soszynska, Marta; Komorowski, Michal; Lewandowska, Paulina; Zdanowski, Robert; Sobiepanek, Anna; Brytan, Marek; Malejczyk, Jacek; Lutynska, Anna; Sciezynska, Aneta.
Affiliation
  • Luszczynski K; Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, 128 Szaserów Street, 04-141 Warsaw, Poland.
  • Soszynska M; Department of Histology and Embryology, Medical University of Warsaw, 02-004 Warsaw, Poland.
  • Komorowski M; Department of Histology and Embryology, Medical University of Warsaw, 02-004 Warsaw, Poland.
  • Lewandowska P; Department of Histology and Embryology, Medical University of Warsaw, 02-004 Warsaw, Poland.
  • Zdanowski R; Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, 128 Szaserów Street, 04-141 Warsaw, Poland.
  • Sobiepanek A; Chair of Drug and Cosmetics Biotechnology, Faculty of Chemistry, Warsaw University of Technology, 00-664 Warsaw, Poland.
  • Brytan M; Department of Pharmacology and Toxicology, Military Institute of Hygiene and Epidemiology, 01-163 Warsaw, Poland.
  • Malejczyk J; Department of Histology and Embryology, Medical University of Warsaw, 02-004 Warsaw, Poland.
  • Lutynska A; Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, 128 Szaserów Street, 04-141 Warsaw, Poland.
  • Sciezynska A; Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, 128 Szaserów Street, 04-141 Warsaw, Poland.
Cells ; 13(14)2024 Jul 17.
Article in En | MEDLINE | ID: mdl-39056788
ABSTRACT
Fibroblasts are among the most abundant cell types in the human body, playing crucial roles in numerous physiological processes, including the structural maintenance of the dermis, production of extracellular matrix components, and mediation of inflammatory responses. Despite their importance, fibroblasts remain one of the least characterized cell populations. The advent of single-cell analysis techniques, particularly single-cell RNA sequencing (scRNA-seq) and fluorescence-activated cell sorting (FACS), has enabled detailed investigations into fibroblast biology. In this study, we present an extensive analysis of fibroblast surface markers suitable for cell sorting and subsequent functional studies. We reviewed over three thousand research articles describing fibroblast populations and their markers, characterizing and comparing subtypes based on their surface markers, as well as their intra- and extracellular proteins. Our detailed analysis identified a variety of distinct fibroblast subpopulations, each with unique markers, characteristics dependent on their location, and the physiological or pathophysiological environment. These findings underscore the diversity of fibroblasts as a cellular population and could lead to the development of novel diagnostic and therapeutic tools.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biomarkers / Cell Separation / Fibroblasts / Flow Cytometry Limits: Animals / Humans Language: En Journal: Cells Year: 2024 Document type: Article Affiliation country: Polonia Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biomarkers / Cell Separation / Fibroblasts / Flow Cytometry Limits: Animals / Humans Language: En Journal: Cells Year: 2024 Document type: Article Affiliation country: Polonia Country of publication: Suiza