Eâ
¡B Mutation Reduces the Pathogenicity of Listeria monocytogenes by Negatively Regulating Biofilm Formation Ability, Infective Capacity, and Virulence Gene Expression.
Vet Sci
; 11(7)2024 Jul 02.
Article
in En
| MEDLINE
| ID: mdl-39057985
ABSTRACT
To explore the role of the membrane permease â
¡B (Eâ
¡B) gene of Listeria pathogenicity island 4 (LIPI-4) in the virulence of Listeria monocytogenes, both an Eâ
¡B deletion strain (∆Eâ
¡B) and a complemented strain were constructed. In vitro experiments demonstrated that Eâ
¡B deletion affected the biofilm formation ability of the wild-type strain (Lm928). Moreover, this deletion decreased the intracellular proliferation abilities of L. monocytogenes. Mice infected with ∆Eâ
¡B survived longer and experienced less weight loss on days 1, 2, and 3 post-infection. The bacterial load in the liver tissue of ∆Eâ
¡B-infected mice was significantly reduced, and a considerable decrease in the blood levels of inflammatory cytokines IL-ß, IL-6, IL-10, and TNF-α were observed. Following Eâ
¡B deletion, 65% (13/20) of genes were downregulated, 25% (5/20) were upregulated, and 10% (2/20) showed no change. These findings suggest that Eâ
¡B deletion may reduce both the in vivo and in vitro virulence levels as well as the biofilm formation ability of Lm928 by downregulating the transcription levels of genes associated with virulence and biofilm formation. These findings provide a foundation for further examining the pathogenic mechanisms of LIPI-4 and Eâ
¡B in L. monocytogenes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Vet Sci
Year:
2024
Document type:
Article
Affiliation country:
China