Your browser doesn't support javascript.
loading
Microbiological and Imaging-Based Evaluations of Photodynamic Therapy Combined with Er:YAG Laser Therapy in the In Vitro Decontamination of Titanium and Zirconia Surfaces.
Munteanu, Ioana-Roxana; Luca, Ruxandra-Elena; Hogea, Elena; Erdelyi, Ralph-Alexandru; Duma, Virgil-Florin; Marsavina, Liviu; Globasu, Amelia-Larisa; Constantin, George-Dumitru; Todea, Darinca Carmen.
Affiliation
  • Munteanu IR; University Clinic of Oral Rehabilitation and Dental Emergencies, Faculty of Dental Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania.
  • Luca RE; Interdisciplinary Research Center for Dental Medical Research, Lasers and Innovative Technologies, 300070 Timisoara, Romania.
  • Hogea E; University Clinic of Oral Rehabilitation and Dental Emergencies, Faculty of Dental Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania.
  • Erdelyi RA; Interdisciplinary Research Center for Dental Medical Research, Lasers and Innovative Technologies, 300070 Timisoara, Romania.
  • Duma VF; Department XIV, Discipline of Microbiology-Virology, Faculty of General Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania.
  • Marsavina L; Department of Measurements and Optical Electronics, Faculty of Electronics, Telecommunications and Information Technology, Polytechnic University Timisoara, 300006 Timisoara, Romania.
  • Globasu AL; Department of Measurements and Optical Electronics, Faculty of Electronics, Telecommunications and Information Technology, Polytechnic University Timisoara, 300006 Timisoara, Romania.
  • Constantin GD; Center of Research and Development for Mechatronics, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, Romania.
  • Todea DC; 3OM Optomechatronics Group, Faculty of Engineering, "Aurel Vlaicu" University of Arad, 310177 Arad, Romania.
Microorganisms ; 12(7)2024 Jun 30.
Article in En | MEDLINE | ID: mdl-39065113
ABSTRACT
The oral cavity's soft and hard tissues create a conducive environment for microbial proliferation and biofilm development, facilitating the colonization of prosthodontic and implant materials such as titanium (Ti) and zirconia (Zr). This study aimed to compare the efficacy of conventional decontamination methodologies (i.e., chemical and mechanical, using 0.12% digluconate chlorhexidine (CHX) solution-treatment and airflow) to adjunctive laser-based interventions on Ti and Zr substrates inoculated with Staphylococcus (S.) aureus ATCC 25923. Additionally, this investigation sought to elucidate the impact of these treatments on temperature variations and surface integrity, analyzing the laser irradiation effects on these prevalent dental materials. Experimental configurations were delineated for both Ti and Zr samples across four groups (1) a conventional treatment group (CV); (2) a photodynamic therapy group (PDT); (3) an ErYAG laser treatment group (Er); (4) a combined PDT and ErYAG treatment group (PDTEr). Also, a negative control group (C) that received no treatment was considered. The decontamination of the inoculated disc samples was evaluated by quantifying the microbial colonies in colony-forming units per milliliter (CFU/mL). Temperature variations on the surface of the samples were determined during laser treatments. Surface modifications were investigated using scanning electron microscopy (SEM) and optical coherence tomography (OCT). For statistical analysis, Fisher 95% confidence intervals, Hsu's MCB method, and the Kruskal-Wallis test were applied. With regard to the 105 CFU/mL of the negative control group, results indicated average values equal for each study group to (1) 2.66 CFU/mL for Ti and 2 CFU/mL for Zr for the CV group; (2) 0.33 CFU/mL for Ti and 1 CFU/mL for Zr for the PDT group; (3) 1.25 CFU/mL for Ti and 0 CFU/mL for Zr for the Er group; (4), and 0 CFU/mL for both Ti and Zr for the PDTEr group. Therefore, the combined PDT and ErYAG treatment (PDTEr) and the singular PDT modality outperformed conventional decontamination methods in eradicating S. aureus biofilms from both Ti and Zr surfaces. Notably, the PDTEr regime achieved a comprehensive elimination of microbial colonies on treated substrates. Surface examination employing OCT demonstrated discernible alterations in the surface morphology of samples subjected to ErYAG and combined PDT and ErYAG treatments. Temperature checks during treatments showed no major changes, suggesting the applied laser methods are safe. In conclusion, PDTEr and PDT eliminated bacteria more effectively, but Zr surfaces were more resilient, making them better for microbe-controlling applications. Also, the study demonstrated that the (less costly but lower resolution) OCT method can replace SEM for such investigations.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Microorganisms Year: 2024 Document type: Article Affiliation country: Rumanía Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Microorganisms Year: 2024 Document type: Article Affiliation country: Rumanía Country of publication: Suiza