Biomarkers for Oral Squamous Cell Carcinoma (miR-24, miR-200, and miR-34): Screening and Detection MicroRNA.
Asian Pac J Cancer Prev
; 25(7): 2265-2269, 2024 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-39068557
ABSTRACT
INTRODUCTION:
Oral squamous cell carcinoma (OSCC) includes about 90% of all oral malignant tumors, and most of them are diagnosed in advanced stages. This study investigated the expression changes of miR-24, miR-200, and miR-34 in saliva samples of patients with oral squamous cell carcinoma, for early diagnosis.METHODS:
In this study, 30 patients and 30 healthy individuals were selected. After RNA extraction and cDNA synthesis, the expression levels of miR-24, miR-200, and miR-34 in saliva samples were measured and evaluated using the Real-Time PCR technique.RESULTS:
Folding change calculation using 2^(-∆∆ Ct) refers to the relative difference in the expression of the markers of the two groups. The expression level of two biomarkers, miR-200 and miR-34, is decreased in patients compared to healthy people; and the expression level of miR-24 is increased in patients compared to healthy people.CONCLUSION:
In general, considering the availability and convenience of saliva sample collection for early detection of the disease, this research result can be considered a diagnostic screening test. To further prove the research results, conducting more extensive studies with more samples is recommended.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saliva
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Biomarkers, Tumor
/
MicroRNAs
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Asian Pac J Cancer Prev
Journal subject:
NEOPLASIAS
Year:
2024
Document type:
Article
Affiliation country:
Irán
Country of publication:
Tailandia