In vitrodegradation of a chitosan-based osteochondral construct points to a transient effect on cellular viability.
Biomed Mater
; 19(5)2024 Aug 06.
Article
in En
| MEDLINE
| ID: mdl-39105245
ABSTRACT
Bioresorbable chitosan scaffolds have shown potential for osteochondral repair applications. Thein vivodegradation of chitosan, mediated by lysozyme and releasing glucosamine, enables progressive replacement by ingrowing tissue. Here the degradation process of a chitosan-nHA based bioresorbable scaffold was investigated for mass loss, mechanical properties and degradation products released from the scaffold when subjected to clinically relevant enzyme concentrations. The scaffold showed accelerated mass loss during the early stages of degradation but without substantial reduction in mechanical strength or structure deterioration. Although not cytotoxic, the medium in which the scaffold was degraded for over 2 weeks showed a transient decrease in mesenchymal stem cell viability, and the main degradation product (glucosamine) demonstrated a possible adverse effect on viability when added at its peak concentration. This study has implications for the design and biomedical application of chitosan scaffolds, underlining the importance of modelling degradation products to determine suitability for clinical translation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Materials Testing
/
Cell Survival
/
Tissue Engineering
/
Chitosan
/
Tissue Scaffolds
/
Mesenchymal Stem Cells
Limits:
Animals
/
Humans
Language:
En
Journal:
Biomed Mater
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2024
Document type:
Article
Affiliation country:
Reino Unido
Country of publication:
Reino Unido