An acridone based fluorescent dye for lipid droplet tracking and cancer diagnosis.
J Photochem Photobiol B
; 258: 113000, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-39121718
ABSTRACT
Lipid droplets (LDs) are spherical organelles that localize in the cytosol of eukaryotic cells. Different proteins are embedded on the surface of LDs, so LDs play a vital role in the physiological activities of cells. The dysregulation of LDs is associated with various human diseases, such as diabetes and obesity. Therefore, it is essential to develop a fluorescent dye that labels LDs to detect and monitor illnesses. In this study, we developed the compound BDAA12C for staining LDs in cells. BDAA12C exhibits excellent LD specificity and low toxicity, enabling us to successfully stain and observe the fusion of LDs in A549 cancer cells. Furthermore, we also successfully distinguished A549 cancer cells and MRC-5 normal cells in a co-culture experiment and in normal and tumour tissues. Interestingly, we found different localizations of BDAA12C in well-fed and starved A549 cancer cells and consequently illustrated the transfer of fatty acids (FAs) from LDs to mitochondria to supply energy for ß-oxidation upon starvation. Therefore, BDAA12C is a promising LD-targeted probe for cancer diagnosis and tracking lipid trafficking within cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lipid Droplets
/
Fluorescent Dyes
Limits:
Humans
Language:
En
Journal:
J Photochem Photobiol B
Journal subject:
BIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Taiwán
Country of publication:
Suiza