A high-performance anion-exchange chromatographic method for the fast analysis and precise determination of varied glucose-derived acids during biomass biorefinery.
Food Chem
; 460(Pt 3): 140626, 2024 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-39128363
ABSTRACT
Glucose-derived acids for the further production of value-added medicine, food additives, and polymers, will promote lignocellulosic biomass biorefinery industry. In response to the diversity and complexity, a new method was established by employing high performance anion exchange chromatography (HPAEC) coupled with a CarboPac™ PA200 column, for the precise and fast determination of glucose, gluconic acid, glucuronic acid, 2-ketogluconic acid, 5-ketogluconic acid and glucaric acid. Based on the analysis of tiny varieties in retention behavior, a gradient elution mode was designed and optimized for the quantitative and qualitative analysis. The protocol displayed acceptable linearity (R2 ≥ 0.995), commendable average recovery rate (95.28% â¼ 99.89%), satisfactory precision (RSD% ≤ 1.5%), and sufficient resolution (R > 6). Additionally, this method was successfully applied to the high-value biorefining process, which confirmed the practicability and accuracy. The results demonstrated that HPAEC has good detection performance for glucose and its derivative acids, and provide key identification technical support for the high-value utilization of lignocellulose.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biomass
/
Glucose
Language:
En
Journal:
Food Chem
/
Food chem
/
Food chemistry
Year:
2024
Document type:
Article
Country of publication:
Reino Unido