Sub-Zero Non-Freezing of Vascularized Composite Allografts in a Rodent Partial Hindlimb Model.
Cryobiology
; : 104950, 2024 Aug 10.
Article
in En
| MEDLINE
| ID: mdl-39134131
ABSTRACT
Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4-6 hours of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore, this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by a storage solution with cryoprotective agents (CPA) developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with modified Steen. This study shows a robust VCA supercooling preservation protocol in a rodent model. Further optimization is expected to allow for its application in a transplantation model, which would be a breakthrough in the field of VCA preservation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Cryobiology
Year:
2024
Document type:
Article