Protocol for analyzing BCG-induced trained immunity in murine bone marrow-derived macrophages.
STAR Protoc
; 5(3): 103267, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-39154346
ABSTRACT
Bacillus Calmette-Guérin (BCG), the only licensed tuberculosis vaccine, provides non-specific protection against non-tuberculosis diseases that is mediated by trained immunity, a functional reprogramming mediated by innate immune memory. Here, we present a protocol for analyzing BCG-induced trained immunity in murine bone marrow-derived macrophages (BMDMs). We describe steps for preparing BCG single bacterial suspensions, isolating BMDM cells, and the training process. This protocol can assist researchers to conveniently utilize BMDM cells to study trained immunity. For complete details on the use and execution of this protocol, please refer to Xu et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
BCG Vaccine
/
Macrophages
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Estados Unidos