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Protocol for analyzing BCG-induced trained immunity in murine bone marrow-derived macrophages.
Xu, Jin-Chuan; Hu, Zhidong; Fan, Xiao-Yong.
Affiliation
  • Xu JC; Shanghai Institute of Infectious Diseases and Biosecurity & Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
  • Hu Z; Shanghai Institute of Infectious Diseases and Biosecurity & Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China. Electronic address: huzhidong@fudan.edu.cn.
  • Fan XY; Shanghai Institute of Infectious Diseases and Biosecurity & Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China. Electronic address: xyfan008@fudan.edu.cn.
STAR Protoc ; 5(3): 103267, 2024 Sep 20.
Article in En | MEDLINE | ID: mdl-39154346
ABSTRACT
Bacillus Calmette-Guérin (BCG), the only licensed tuberculosis vaccine, provides non-specific protection against non-tuberculosis diseases that is mediated by trained immunity, a functional reprogramming mediated by innate immune memory. Here, we present a protocol for analyzing BCG-induced trained immunity in murine bone marrow-derived macrophages (BMDMs). We describe steps for preparing BCG single bacterial suspensions, isolating BMDM cells, and the training process. This protocol can assist researchers to conveniently utilize BMDM cells to study trained immunity. For complete details on the use and execution of this protocol, please refer to Xu et al.1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: BCG Vaccine / Macrophages Limits: Animals Language: En Journal: STAR Protoc Year: 2024 Document type: Article Affiliation country: China Country of publication: Estados Unidos

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: BCG Vaccine / Macrophages Limits: Animals Language: En Journal: STAR Protoc Year: 2024 Document type: Article Affiliation country: China Country of publication: Estados Unidos