Establishment and Validation of a Multiplex PCR Detection System for the Identification of Six Common Edible Meat Components.
Fa Yi Xue Za Zhi
; 40(3): 254-260, 2024 Jun 25.
Article
in En, Zh
| MEDLINE
| ID: mdl-39166306
ABSTRACT
OBJECTIVES:
To establish a rapid, accurate, and sensitive multiplex PCR detection method for the simultaneous identification of the six common edible meats (beef, lamp, chicken, pork, goose, duck), and to evaluate its application value in meat adulteration identification.METHODS:
Based on complete mitochondrial genomic sequences of six species in the GenBank database, DNA sequences (cattleï¼16S rRNA; sheepï¼COX-1; chickensï¼Cytb; pigï¼COX-1; gooseï¼NADH2; duckï¼16S rRNA) with intra-species conservation and inter-species specificity were screened, and species-specific primers were designed to construct a multiplex PCR detection system that can simultaneously detect the meat of six common species. The species specificity, sensitivity and reproducibility of the system were studied, and the simulated mixture sample detection was performed.RESULTS:
This study successfully constructed a multiplex PCR detection system that can detect the meats of six common species simultaneously. The system was not effective in DNA amplification of non-target species. When the DNA template sizes were 0.062 5-2 ng/µL, the amplified products of all six species could be detected. The duck component was still detected when the mixing ratio of duck and beef was as low as 0.5%.CONCLUSIONS:
This study constructs and establishes a multiplex PCR detection system with strong specificity, high sensitivity, and good reproducibility. It can accurately identify the components of animal origin in common edible meats and provide a simple and practical method for identifying adulteration of common edible meats and meat products in China.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Species Specificity
/
Chickens
/
Sensitivity and Specificity
/
DNA Primers
/
Ducks
/
Multiplex Polymerase Chain Reaction
/
Geese
/
Meat
Limits:
Animals
Language:
En
/
Zh
Journal:
Fa Yi Xue Za Zhi
/
Fa yi xue za zhi
Journal subject:
JURISPRUDENCIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
China