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Transcriptome analysis of Drosophila suzukii reveals molecular mechanisms conferring pyrethroid and spinosad resistance.
Tabuloc, Christine A; Carlson, Curtis R; Ganjisaffar, Fatemeh; Truong, Cindy C; Chen, Ching-Hsuan; Lewald, Kyle M; Hidalgo, Sergio; Nicola, Nicole L; Jones, Cera E; Sial, Ashfaq A; Zalom, Frank G; Chiu, Joanna C.
Affiliation
  • Tabuloc CA; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Carlson CR; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Ganjisaffar F; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Truong CC; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Chen CH; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Lewald KM; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Hidalgo S; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Nicola NL; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Jones CE; Department of Entomology, University of Georgia, Athens, GA, USA.
  • Sial AA; Department of Entomology, University of Georgia, Athens, GA, USA.
  • Zalom FG; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA.
  • Chiu JC; Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, California, USA. jcchiu@ucdavis.edu.
Sci Rep ; 14(1): 19867, 2024 08 27.
Article in En | MEDLINE | ID: mdl-39191909
ABSTRACT
Drosophila suzukii lay eggs in soft-skinned, ripening fruits, making this insect a serious threat to berry production. Since its 2008 introduction into North America, growers have used insecticides, such as pyrethroids and spinosads, as the primary approach for D. suzukii management, resulting in development of insecticide resistance in this pest. This study sought to identify the molecular mechanisms conferring insecticide resistance in these populations. We sequenced the transcriptomes of two pyrethroid- and two spinosad-resistant isofemale lines. In both pyrethroid-resistant lines and one spinosad-resistant line, we identified overexpression of metabolic genes that are implicated in resistance in other insect pests. In the other spinosad-resistant line, we observed an overexpression of cuticular genes that have been linked to resistance. Our findings enabled the development of molecular diagnostics that we used to confirm persistence of insecticide resistance in California, U.S.A. To validate these findings, we leveraged D. melanogaster mutants with reduced expression of metabolic or cuticular genes that were found to be upregulated in resistant D. suzukii to demonstrate that these genes are involved in promoting resistance. This study is the first to characterize the molecular mechanisms of insecticide resistance in D. suzukii and provides insights into how current management practices can be optimized.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyrethrins / Insecticide Resistance / Macrolides / Gene Expression Profiling / Drosophila / Drug Combinations / Insecticides Limits: Animals Language: En Journal: Sci Rep Year: 2024 Document type: Article Affiliation country: Estados Unidos Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyrethrins / Insecticide Resistance / Macrolides / Gene Expression Profiling / Drosophila / Drug Combinations / Insecticides Limits: Animals Language: En Journal: Sci Rep Year: 2024 Document type: Article Affiliation country: Estados Unidos Country of publication: Reino Unido