Mechanism of Stimulation of Myogenesis under the Action of Succinic Acid through the Succinate Receptor SUCNR1.
Biochemistry (Mosc)
; 89(7): 1325-1335, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-39218028
ABSTRACT
Effect of succinic acid on the processes of myogenesis was investigated in the study with the cells of C2C12 line. In the concentration range 10-1000 µM, succinic acid stimulated the process of myogenic differentiation, increasing the levels of myogenesis factors MyoD (at all stages of myogenesis) and myogenin (at the stage of terminal differentiation). Presence of the succinate receptors SUCNR1 was revealed in the C2C12 cells using Western blotting, level of which decreased during myogenesis. When succinic acid was added to the cells, the level of intracellular succinate did not change significantly and decreased during myogenic differentiation. Using a specific Gai protein inhibitor, pertussis toxin, it was found that stimulation of myogenesis in the C2C12 cells under the action of succinic acid is realized through SUCNR1-Gai interaction.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Succinic Acid
/
Muscle Development
Limits:
Animals
Language:
En
Journal:
Biochemistry (Mosc)
Year:
2024
Document type:
Article
Affiliation country:
Rusia
Country of publication:
Estados Unidos