Controlled preparation of tannic acid-derived carbonized dots and their use to inhibit amyloid aggregation and promote aggregate disaggregation.
Mikrochim Acta
; 191(10): 573, 2024 09 04.
Article
in En
| MEDLINE
| ID: mdl-39227417
ABSTRACT
Tannic acid (TA)-derived carbon dots (TACDs) were synthesized for the first time via a solvothermal method using TA as one of the raw materials, which may effectively inhibit amyloid fibril aggregation and disaggregate mature fibril. The fluorescent property of TACDs were modulated by adjusting the ratio of TA to o-phenylenediamine (oPD), and TACDs fabricated with the precursor ratio as 11 showed the best fluorescent property. Circular dichroism spectra (CD) showed that the structure of ß-sheet decreased as the concentration of TACDs increased. The inhibition efficiency, as confirmed by thioflavin T (ThT) and transmission electron microscopy (TEM), is extraordinary at 98.16%, whereas disaggregation efficiency is noteworthy at 97.97%, and the disaggregated lysozyme fibrils did not reaggregate after 7 days. More critically, TACDs can also alleviate the cellular toxicity caused by Aß fibrils and improve cell viability. This work offers a new perspective on the design of scavengers for amyloid plaques.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tannins
/
Carbon
/
Protein Aggregates
Limits:
Animals
/
Humans
Language:
En
Journal:
Mikrochim Acta
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Austria