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Achieving optical transparency in live animals with absorbing molecules.
Ou, Zihao; Duh, Yi-Shiou; Rommelfanger, Nicholas J; Keck, Carl H C; Jiang, Shan; Brinson, Kenneth; Zhao, Su; Schmidt, Elizabeth L; Wu, Xiang; Yang, Fan; Cai, Betty; Cui, Han; Qi, Wei; Wu, Shifu; Tantry, Adarsh; Roth, Richard; Ding, Jun; Chen, Xiaoke; Kaltschmidt, Julia A; Brongersma, Mark L; Hong, Guosong.
Affiliation
  • Ou Z; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Duh YS; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Rommelfanger NJ; Department of Physics, Stanford University, Stanford, CA, USA.
  • Keck CHC; Geballe Laboratory for Advanced Materials, Stanford University, Stanford, CA, USA.
  • Jiang S; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Brinson K; Department of Applied Physics, Stanford University, Stanford, CA, USA.
  • Zhao S; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Schmidt EL; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Wu X; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Yang F; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Cai B; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Cui H; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Qi W; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Wu S; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Tantry A; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Roth R; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Ding J; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Chen X; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Kaltschmidt JA; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
  • Brongersma ML; Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA, USA.
  • Hong G; Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Science ; 385(6713): eadm6869, 2024 Sep 06.
Article in En | MEDLINE | ID: mdl-39236186
ABSTRACT
Optical imaging plays a central role in biology and medicine but is hindered by light scattering in live tissue. We report the counterintuitive observation that strongly absorbing molecules can achieve optical transparency in live animals. We explored the physics behind this observation and found that when strongly absorbing molecules dissolve in water, they can modify the refractive index of the aqueous medium through the Kramers-Kronig relations to match that of high-index tissue components such as lipids. We have demonstrated that our straightforward approach can reversibly render a live mouse body transparent to allow visualization of a wide range of deep-seated structures and activities. This work suggests that the search for high-performance optical clearing agents should focus on strongly absorbing molecules.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Optical Imaging Limits: Animals Language: En Journal: Sci. (N.Y., N.Y.) / Science Year: 2024 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Optical Imaging Limits: Animals Language: En Journal: Sci. (N.Y., N.Y.) / Science Year: 2024 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos