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Detection of Genes Related to Antibiotic Resistance in Leptospira.
Pineda, Santiago; Martínez Garro, Juliana María; Salazar Flórez, Jorge Emilio; Agudelo-Pérez, Sergio; Monroy, Fernando P; Peláez Sánchez, Ronald Guillermo.
Affiliation
  • Pineda S; CES Biology, Science and Biotechnology School, CES University, Medellin 050021, Colombia.
  • Martínez Garro JM; CES Biology, Science and Biotechnology School, CES University, Medellin 050021, Colombia.
  • Salazar Flórez JE; Medicine Program, GEINCRO Research Group, School of Health Sciences, San Martín University Foundation, Sabaneta 055450, Colombia.
  • Agudelo-Pérez S; Department of Pediatrics, Medicine School, Universidad de La Sabana, Chía 025001, Colombia.
  • Monroy FP; Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.
  • Peláez Sánchez RG; Life and Health Sciences Research Group, Graduate School, CES University, Medellin 050021, Colombia.
Trop Med Infect Dis ; 9(9)2024 Sep 06.
Article in En | MEDLINE | ID: mdl-39330892
ABSTRACT
Leptospirosis is a disease caused by the bacteria of the Leptospira genus, which can usually be acquired by humans through contact with urine from infected animals; it is also possible for this urine to contaminate soils and bodies of water. The disease can have deadly consequences in some extreme cases. Fortunately, until now, patients with leptospirosis have responded adequately to treatment with doxycycline and azithromycin, and no cases of antibiotic resistance have been reported. However, with the extensive use of such medications, more bacteria, such as Staphylococci and Enterococci, are becoming resistant. The purpose of this study is to determine the presence of genes related to antibiotic resistance in the Leptospira genus using bioinformatic tools, which have not been undertaken in the past. Whole genomes from the 69 described Leptospira species were downloaded from NCBI's GeneBank and analyzed using CARD (The Comprehensive Antibiotic Resistant Database) and RAST (Rapid Annotations using Subsystem Technology). After a detailed genomic search, 12 genes associated with four mechanisms were found resistance to beta-lactamases, vancomycin, aminoglycoside adenylyltransferases, as well as multiple drug efflux pumps. Some of these genes are highly polymorphic among different species, and some of them are present in multiple copies in the same species. In conclusion, this study provides evidence of the presence of genes related to antibiotic resistance in the genomes of some species of the genus Leptospira, and it is the starting point for future experimental evaluation to determine whether these genes are transcriptionally active in some species and serovars.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Trop Med Infect Dis Year: 2024 Document type: Article Affiliation country: Colombia Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Trop Med Infect Dis Year: 2024 Document type: Article Affiliation country: Colombia Country of publication: Suiza