Effects of tunicamycin on the hypothalamo-neurohypophysial system of the rat.
Cell Tissue Res
; 217(1): 199-210, 1981.
Article
in En
| MEDLINE
| ID: mdl-7249043
ABSTRACT
Intracisternal injections of tunicamycin, an inhibitor of glycosylation, decreased the incorporation of [35S]cysteine into the neurophysins in the rat neurohypophysis. Histochemical and immunocytochemical studies showed that there was no concomitant decrease in the amount of secretory product in the perikarya of the hypothalamo-neurohypophysial neurones. Indeed there was an increase, although this was not associated with neurosecretory granules as judged electron-microscopically. Tunicamycin led to the formation of so-called "colloid droplets" which were immunopositive and of which the ultrastructural correlates appeared to be product-filled dilatations of the rough endoplasmic reticulum. The observations are interpreted to suggest that glycosylation plays a rôle in the packaging of secretory material in the hypothalamo-neurohypophysial system.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pituitary Gland, Posterior
/
Pituitary Hormones, Posterior
/
Supraoptic Nucleus
/
Neurophysins
/
Tunicamycin
/
Glucosamine
/
Hypothalamus
Limits:
Animals
Language:
En
Journal:
Cell Tissue Res
Year:
1981
Document type:
Article