Expression patterns of predicted genes from the C. elegans genome sequence visualized by FISH in whole organisms.
Nat Genet
; 11(3): 314-20, 1995 Nov.
Article
in En
| MEDLINE
| ID: mdl-7581456
ABSTRACT
More than 10 megabases of contiguous genome sequence have been submitted to the databases by the Caenorhabditis elegans Genome Sequencing Consortium. To characterize the genes predicted from the sequence, we have developed high resolution FISH for visualization of mRNA distributions in whole animals. The high resolution and sensitivity afforded by the use of directly fluorescently labelled probes and confocal imaging permitted mRNA distributions to be recorded at the cellular and subcellular level. Expression patterns were obtained for 8 out of 10 genes in an initial test set of predicted gene sequences, indicating that FISH is an effective means of characterizing predicted genes in C. elegans.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Caenorhabditis
/
Gene Expression
/
Genome
/
In Situ Hybridization, Fluorescence
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Nat Genet
Journal subject:
GENETICA MEDICA
Year:
1995
Document type:
Article
Affiliation country:
Reino Unido