Gas-liquid chromatographic determination of mono- and diiodotyrosines in serum.
Clin Chem
; 25(2): 218-20, 1979 Feb.
Article
in En
| MEDLINE
| ID: mdl-759018
A sensitivie, reliable gas-chromatographic assay for monoiodotyrosine and diiodotyrosine in human serum is reported. The oxazolidinone-heptafluorobutyric anhydride derivatives allow the quantitation of both compounds in the linear range of 0.2 to 7.6 mg/L of serum. Analytical recovery averaged 88%, and mean accuracy and within-run precision were 98 and 2%, respectively. Concentrations of monoiodotyrosine in serum as low as 20 microgram/L and of diiodotyrosine as low as 100 microgram/L can be detected. Normal serum contains no detectable concentration of either compound, but the method is applicable as a diagnostic tool in the early prediction of thyroid disease. Both compounds were detected in the serum of a hypothyroid subject whose normal thyroid hormone concentrations were being maintained by therapy with desiccated thyroid extract.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Diiodotyrosine
/
Monoiodotyrosine
Limits:
Humans
Language:
En
Journal:
Clin Chem
Journal subject:
QUIMICA CLINICA
Year:
1979
Document type:
Article
Country of publication:
Reino Unido