Sources of human Schwann cells and the influence of donor age.
Exp Neurol
; 130(1): 53-5, 1994 Nov.
Article
in En
| MEDLINE
| ID: mdl-7821396
ABSTRACT
We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 x 10(4) and 2.9 x 10(4), respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 x 10(4)). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schwann Cells
/
Tissue Donors
/
Aging
Limits:
Adult
/
Aged
/
Child
/
Child, preschool
/
Humans
/
Middle aged
Language:
En
Journal:
Exp Neurol
Year:
1994
Document type:
Article