The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH.
EMBO J
; 14(4): 779-90, 1995 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-7882981
ABSTRACT
The pH regulation of gene expression in Aspergillus nidulans is mediated by pacC, whose 678 residue-derived protein contains three putative Cys2His2 zinc fingers. Ten pacCc mutations mimicking growth at alkaline pH remove between 100 and 214 C-terminal residues, including a highly acidic region containing an acidic glutamine repeat. Nine pacC+/- mutations mimicking acidic growth conditions remove between 299 and 505 C-terminal residues. Deletion of the entire pacC coding region mimics acidity but leads additionally to poor growth and conidiation. A PacC fusion protein binds DNA with the core consensus GCCARG. At alkaline ambient pH, PacC activates transcription of alkaline-expressed genes (including pacC itself) and represses transcription of acid-expressed genes. pacCc mutations obviate the need for pH signal transduction.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aspergillus nidulans
/
Acid-Base Equilibrium
/
Transcription Factors
/
Fungal Proteins
/
DNA-Binding Proteins
Language:
En
Journal:
EMBO J
Year:
1995
Document type:
Article
Affiliation country:
Reino Unido