Determination of a complete set of coupling constants in 13C-labeled oligonucleotides.
J Biomol NMR
; 4(5): 631-44, 1994 Sep.
Article
in En
| MEDLINE
| ID: mdl-7919950
ABSTRACT
Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligoribonucleotides
/
Magnetic Resonance Spectroscopy
/
Nucleic Acid Conformation
Language:
En
Journal:
J Biomol NMR
Journal subject:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
/
MEDICINA NUCLEAR
Year:
1994
Document type:
Article
Affiliation country:
Alemania