A high-sensitivity electrochemiluminescence-based detection system for automated PCR product quantitation.
Biotechniques
; 15(1): 152-7, 1993 Jul.
Article
in En
| MEDLINE
| ID: mdl-8363832
ABSTRACT
A high-sensitivity nonisotopic system has been developed for post-PCR product detection. The probe-based detection system exploits a chemiluminescent reaction that takes place on the electrode surface in an electrochemical cell. The detection system incorporates a biotin-streptavidin capture reaction onto a solid support that permits fast post-PCR product detection at the attomole level. The system precision is within 5% relative standard deviation over a linear dynamic range of greater than three orders of magnitude. In this paper, the principles and features of the electrochemiluminescent-based detection system, together with its application to PCR product quantitation, are described in detail.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autoanalysis
/
Polymerase Chain Reaction
/
Luminescent Measurements
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Biotechniques
Year:
1993
Document type:
Article