Nucleosomal organization of telomere-specific chromatin in rat.
Cell
; 73(4): 775-87, 1993 May 21.
Article
in En
| MEDLINE
| ID: mdl-8500170
ABSTRACT
Rat liver interphase chromosomes have telomeres 20-100 kb in length. Micrococcal nuclease digestion of nuclei cleaves telomeres with a uniform 157 bp periodicity, producing soluble particles that sediment in sucrose gradients exactly like oligonucleosomes. The monomeric telomere particles comigrate with nucleosome core particles on nucleoprotein and DNA gels but do not bind H1. DNAase I cleaves telomere nucleoprotein into a series of bands spaced by about 10.4 bp and with the same intensity distribution as bands from bulk nucleosomes. Removal of H1 from chromatin alters the sedimentation properties of telomeres in parallel with bulk chromatin. Thus, telomeres of mammals are constructed of closely spaced nucleosomes, in contrast with the telomeres of lower eukaryotes, which show no evidence of nucleosomal structure.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleosomes
/
Telomere
Limits:
Animals
Language:
En
Journal:
Cell
Year:
1993
Document type:
Article