Contiguous patches of normal human mammary epithelium derived from a single stem cell: implications for breast carcinogenesis.
Cancer Res
; 56(2): 402-4, 1996 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-8542598
ABSTRACT
Tissue clonality can be assessed in females by analyzing the methylation status of polymorphic DNA markers on X-linked genes because extensive de novo methylation of one allele at the preimplantation stage is associated with its permanent inactivation. We applied X chromosome inactivation toward understanding human breast morphogenesis by examining the nonmalignant breast epithelium from two reduction mammaplasties and a mastectomy. We found that entire lobules and large ducts of normal breast tissue have the same X chromosome inactivated, suggesting that they are derived from the same stem cell. The regions of inactivation of a particular X chromosome do not extend over an entire breast, so that ducts and lobules with opposite chromosomes inactivated are present within a single breast. Potential relevance of these observations for malignant transformation is discussed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
Breast
/
Breast Neoplasms
Limits:
Female
/
Humans
Language:
En
Journal:
Cancer Res
Year:
1996
Document type:
Article
Affiliation country:
Estados Unidos