cDNA cloning and genomic structure of human bone morphogenetic protein-3B (BMP-3b).
Biochem Biophys Res Commun
; 223(2): 304-10, 1996 Jun 14.
Article
in En
| MEDLINE
| ID: mdl-8670277
ABSTRACT
BMP-3b (also termed GDF-10) is a novel BMP-3 related protein recently discovered in rat femur tissue by molecular cloning. In this paper, we have isolated cDNA and the gene for human BMP-3b and determined their structure. Cloned human BMP-3b cDNA with a size of 2632 base pairs encodes a 478 amino acid precursor protein sharing 83% identity with rat BMP-3b. The human BMP-3b gene is composed of 3 exons and spans approximately 13 kilobases of DNA. The 5' flanking region carries no typical TATA box but G+C rich sequences. Southern blot analysis revealed that the BMP-3b gene is situated in a single locus of chromosome 10. By Northern analysis, human BMP-3b transcripts were detected primarily in femur, brain, lung, skeletal muscle, pancreas and testis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromosomes, Human, Pair 10
/
Growth Substances
/
Bone Morphogenetic Proteins
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1996
Document type:
Article
Affiliation country:
Japón