Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage.
Proc Natl Acad Sci U S A
; 93(14): 7125-30, 1996 Jul 09.
Article
in En
| MEDLINE
| ID: mdl-8692956
ABSTRACT
We have used telomeric DNA to break two acrocentric derivatives of the human Y chromosome into mini-chromosomes that are small enough to be size- fractionated by pulsed-field gel electrophoresis. One of the mini-chromosomes is about 7 Mb in size and sequence-tagged site analysis of this molecule suggests that it corresponds to a simple truncation of the short arm of the Y chromosome. Five of the mini-chromosomes are derived from the long arm, are all rearranged by more than a simple truncation, and range in size from 4.0 Mb to 9 Mb. We have studied the mitotic stabilities of these mini-chromosomes and shown that they are stably maintained by cells proliferating in culture for about 100 cell divisions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Y Chromosome
/
Chromosome Aberrations
/
Telomere
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
1996
Document type:
Article
Affiliation country:
Reino Unido