SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.
Cell
; 86(2): 263-74, 1996 Jul 26.
Article
in En
| MEDLINE
| ID: mdl-8706131
ABSTRACT
We have identified the yeast and human homologs of the SKP1 gene as a suppressor of cdc4 mutants and as a cyclin F-binding protein. Skp1p indirectly binds cyclin A/Cdk2 through Skp2p, and directly binds Skp2p, cyclin F, and Cdc4p through a novel structural motif called the F-box. SKP1 is required for ubiquitin-mediated proteolysis of Cin2p, Clb5p, and the Cdk inhibitor Sic1p, and provides a link between these molecules and the proteolysis machinery. A large number of proteins contain the F-box motif and are thereby implicated in the ubiquitin pathway. Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ubiquitins
/
Cell Cycle Proteins
/
Ubiquitin-Protein Ligase Complexes
/
Ubiquitin-Protein Ligases
/
F-Box Proteins
Limits:
Humans
Language:
En
Journal:
Cell
Year:
1996
Document type:
Article
Affiliation country:
Estados Unidos