Structural studies of the O-specific chain of Hafnia alvei strain 32 lipopolysaccharide.
Carbohydr Res
; 292: 117-28, 1996 Oct 04.
Article
in En
| MEDLINE
| ID: mdl-8870241
ABSTRACT
The structure of the O-specific side chain of the Hafnia alvei strain 32 lipopolysaccharide has been investigated. Methylation analysis, partial acid hydrolysis, Smith degradations, NMR spectroscopy, MALDI-TOF and FAB mass spectrometry in combination with collision-induced decomposition MS/MS were the principal methods used. It is concluded that the polysaccharide is composed of pentasaccharide repeating units having the following structure which is partially O-acetylated in the 2- (20%) and 3- (50%) position of the-->4)-alpha-D-GalpA-(1-->residue. [sequencesee text] A MALDI-TOF mass spectrum of the O-specific chains indicated that they consisted of up to 16 repeating units.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
O Antigens
/
Enterobacteriaceae
Language:
En
Journal:
Carbohydr Res
Year:
1996
Document type:
Article
Affiliation country:
Polonia