Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells.
Clin Exp Immunol
; 106(1): 86-90, 1996 Oct.
Article
in En
| MEDLINE
| ID: mdl-8870703
ABSTRACT
Glycophosphatidylinositol (GPI)-linked Thy-1 molecules, well known cell surface markers of murine T cells, are present on the glomerular mesangial cells of the rat kidney. The administration of anti-Thy-1.1 MoAbs 1-22-3 and OX-7 to rats induces severe and mild complement-dependent mesangial proliferative glomerulonephritis, respectively. In order to determine whether protein-tyrosine kinase (PTK) activity is associated with Thy-1 molecules on rat mesangial cell surface, we performed an immune complex kinase assay, using anti-Thy-1 MoAbs 1-22-3 and OX-7, followed by reimmunoprecipitation with anti-phosphotyrosine, anti-fyn, anti-lck and anti-lyn antibodies. Physical association of PTK, p59fyn and p56/53lyn with Thy-1 molecules was demonstrated in cultured rat mesangial cells. The activities of these kinases detected in MoAb 1-22-3 precipitates were higher than those in MoAb OX-7 precipitates. These results suggest that Thy-1 molecule transduces some signals also in rat mesangial cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein-Tyrosine Kinases
/
Thy-1 Antigens
/
Glomerular Mesangium
Type of study:
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Clin Exp Immunol
Year:
1996
Document type:
Article
Affiliation country:
Japón