Regulation of NF-kappaB by cyclin-dependent kinases associated with the p300 coactivator.
Science
; 275(5299): 523-7, 1997 Jan 24.
Article
in En
| MEDLINE
| ID: mdl-8999795
ABSTRACT
The nuclear factor kappaB (NF-kappaB) transcription factor is responsive to specific cytokines and stress and is often activated in association with cell damage and growth arrest in eukaryotes. NF-kappaB is a heterodimeric protein, typically composed of 50- and 65-kilodalton subunits of the Rel family, of which RelA(p65) stimulates transcription of diverse genes. Specific cyclin-dependent kinases (CDKs) were found to regulate transcriptional activation by NF-kappaB through interactions with the coactivator p300. The transcriptional activation domain of RelA(p65) interacted with an amino-terminal region of p300 distinct from a carboxyl-terminal region of p300 required for binding to the cyclin E-Cdk2 complex. The CDK inhibitor p21 or a dominant negative Cdk2, which inhibited p300-associated cyclin E-Cdk2 activity, stimulated kappaB-dependent gene expression, which was also enhanced by expression of p300 in the presence of p21. The interaction of NF-kappaB and CDKs through the p300 and CBP coactivators provides a mechanism for the coordination of transcriptional activation with cell cycle progression.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Nuclear Proteins
/
Transcriptional Activation
/
Trans-Activators
/
NF-kappa B
/
Protein Serine-Threonine Kinases
/
Cyclin-Dependent Kinases
/
CDC2-CDC28 Kinases
Type of study:
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Science
Year:
1997
Document type:
Article
Affiliation country:
Estados Unidos