Calmodulin regulation of calcium stores in phototransduction of Drosophila.
Science
; 275(5303): 1119-21, 1997 Feb 21.
Article
in En
| MEDLINE
| ID: mdl-9027311
ABSTRACT
Phototransduction in Drosophila occurs through the ubiquitous phosphoinositide-mediated signal transduction system. Major unresolved questions in this pathway are the identity and role of the internal calcium stores in light excitation and the mechanism underlying regulation of Ca2+ release from internal stores. Treatment of Drosophila photoreceptors with ryanodine and caffeine disrupted the current induced by light, whereas subsequent application of calcium-calmodulin (Ca-CaM) rescued the inactivated photoresponse. In calcium-deprived wild-type Drosophila and in calmodulin-deficient transgenic flies, the current induced by light was disrupted by a specific inhibitor of Ca-CaM. Furthermore, inhibition of Ca-CaM revealed light-induced release of calcium from intracellular stores. It appears that functional ryanodine-sensitive stores are essential for the photoresponse. Moreover, calcium release from these stores appears to be a component of Drosophila phototransduction, and Ca-CaM regulates this process.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Calmodulin
/
Signal Transduction
/
Calcium
/
Photoreceptor Cells, Invertebrate
/
Light
Limits:
Animals
Language:
En
Journal:
Science
Year:
1997
Document type:
Article
Affiliation country:
Israel