A dual-marker system for quantitative studies of myoblast transplantation in the mouse.
Transplantation
; 63(12): 1794-7, 1997 Jun 27.
Article
in En
| MEDLINE
| ID: mdl-9210506
ABSTRACT
BACKGROUND:
Myoblast transplantation (MT) is a potential approach for gene transfer into skeletal muscle, the efficiency of which depends upon the number of copies of donor genome incorporated into the host tissue. We have developed a system for quantitative studies of MT that measures amounts of donor-derived genome in host muscles and estimates the contributions of donor cell survival and proliferation in vivo.METHODS:
[14C]thymidine-labeled, male myoblasts were transplanted into female muscles, providing two donor cell markers, Y chromosome and [14C]. The markers were measured in muscle extracts by slot blotting and scintillation counting, respectively.RESULTS:
In each extract, the amount of Y chromosome was used to quantify donor-derived genome, whereas the radiolabel provided an estimate of cell survival. Furthermore, the different modes of inheritance of the markers meant that proliferation of surviving donor cells was detected as a change in marker ratio.CONCLUSIONS:
This system provides a method for assessing potential improvements of MT.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Muscle Fibers, Skeletal
Limits:
Animals
Language:
En
Journal:
Transplantation
Year:
1997
Document type:
Article
Affiliation country:
Reino Unido