Efficacy of a subcutaneously administered, ultraviolet light-killed Pasteurella multocida A:3-containing bacterin against transthoracic challenge exposure in goats.
Am J Vet Res
; 58(8): 841-7, 1997 Aug.
Article
in En
| MEDLINE
| ID: mdl-9256967
OBJECTIVE: To determine the effectiveness of Pasteurella multocida biovar A, serovar 3 (Pm A:3) killed by exposure to UV light and incorporated with a polyacrylate bead carrier as a vaccine. ANIMALS: 18 weanling male Spanish goats. PROCEDURE: Prospective, randomized controlled study with 3 treatment groups: positive-control (PC), negative-control (NC), and principal Pm A:3 bacterin (PA) groups. Six PC goats each received live Pm A:3 and polyacrylate beads twice, 22 days apart, by transthoracic injection into the left lung. Six NC goats each received only PA beads twice, 22 days apart, by transthoracic injection. Six principal goats each received Pm A:3 vaccine SC twice, 22 days apart. Fourteen days after the second vaccination, all goats were challenge exposed with live Pm A:3 by transthoracic injection into the right lung, and 4 days later they were euthanatized and necropsied. RESULTS: Mean volume of consolidated lung tissue at the challenge site was 1.75 cm3 for the PC group, 15.18 cm3 for the NC group, and 3.9 cm3 for the PA vaccine group. The NC group had a significantly (P < or = 0.002) larger mean volume of consolidated lung tissue than did the PC and PA groups after challenge exposure. CONCLUSIONS: The PA bacterin and the PC groups developed protective immunity against live Pm A:3 challenge exposure. An SC administered, UV light-killed, Pm A:3 bacterin induced protective immunity similar to that induced by virulent live Pm A:3 injected into the target organ, the lung.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pasteurella Infections
/
Bacterial Vaccines
/
Goat Diseases
/
Pasteurella multocida
Type of study:
Observational_studies
Limits:
Animals
Language:
En
Journal:
Am J Vet Res
Year:
1997
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Estados Unidos