Preliminary crystallographic characterization of ricin agglutinin.
Proteins
; 28(4): 586-9, 1997 Aug.
Article
in En
| MEDLINE
| ID: mdl-9261874
The quaternary structure of ricin agglutinin (RCA) has been determined by x-ray crystallography. The refined structure of ricin proved to be a successful search model using the molecular replacement method of phase determination. RCA forms an elongated molecule of dimensions 120 A x 60 A x 40 A with two A chains at the center and a B chain at each end. The A chains are covalently associated via a disulfide bridge between Cys 156 of both chains. Additional contacts at residues 114-5 stabilize the dimer interface. The covalent association of RCAA chains was confirmed by gel filtration under reducing and nonreducing conditions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Conformation
/
Plant Lectins
/
Lectins
Language:
En
Journal:
Proteins
Journal subject:
BIOQUIMICA
Year:
1997
Document type:
Article
Affiliation country:
Reino Unido
Country of publication:
Estados Unidos