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Valproic acid suppresses G1 phase-dependent sialylation of a 65kDa glycoprotein in the C6 glioma cell cycle.
Bacon, C L; O'Driscoll, E; Regan, C M.
Affiliation
  • Bacon CL; Department of Pharmacology, University College, Belfield, Dublin, Ireland.
Int J Dev Neurosci ; 15(6): 777-84, 1997 Oct.
Article in En | MEDLINE | ID: mdl-9402228
The influence of valproate on in vitro glycosylation events in C6 glioma has been investigated, as this major human teratogen restricts proliferation in the mid-G1 phase of the cycle and alters the prevalence and/or glycosylation state of cell surface glycoproteins with the potential to mediate cell-cell and cell matrix interactions critical to development. C6 glioma cultured continuously in the presence of 1 mM valproate exhibited a significant depression of exponential growth but attained confluency one day later, when the majority of cells entered the G1 phase of the cycle. Glycoprotein sialyltransferase, which exhibited a four-fold increase during exponential growth and a small decrease at confluency, was markedly attenuated in valproate-exposed cells in a manner which was indirect. This was associated with an inhibition of transient alpha2,3 sialylation of a 65 kDa glycoprotein expressed maximally at 4 hr into the G1 phase of the cell cycle. This effect was cell-cycle phase-specific, as exposure of synchronized cells to valproate inhibited transient sialylation at 4 and 5 hr into the G1 phase. Inhibition of the 65 kDa glycoprotein sialylation by valproate is suggested to arise from impaired signal transduction preceding the eventual arrest by the drug at a 5-6 hr G1 phase restriction point.
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Collection: 01-internacional Database: MEDLINE Main subject: Sialyltransferases / Glycoproteins / G1 Phase / Valproic Acid / Enzyme Inhibitors / Glioma Type of study: Risk_factors_studies Limits: Humans Language: En Journal: Int J Dev Neurosci Year: 1997 Document type: Article Affiliation country: Irlanda Country of publication: Estados Unidos
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Collection: 01-internacional Database: MEDLINE Main subject: Sialyltransferases / Glycoproteins / G1 Phase / Valproic Acid / Enzyme Inhibitors / Glioma Type of study: Risk_factors_studies Limits: Humans Language: En Journal: Int J Dev Neurosci Year: 1997 Document type: Article Affiliation country: Irlanda Country of publication: Estados Unidos