Detection of exogenous hydrocortisone in horse urine by gas chromatography-combustion-carbon isotope ratio mass spectrometry.
J Chromatogr B Biomed Sci Appl
; 702(1-2): 85-91, 1997 Nov 21.
Article
in En
| MEDLINE
| ID: mdl-9449559
ABSTRACT
A gas chromatography-combustion-isotope ratio mass spectrometry method for confirmation of hydrocortisone abuse in horseracing and equine sports is proposed. Urinary hydrocortisone was converted to a bismethylenedioxy derivative which presents good gas chromatographic properties and brings an extra carbon contribution of only two carbon atoms. Synthetic hydrocortisone has a different 13C abundance from that of natural urinary horse hydrocortisone and the difference is significant, therefore exogenous and endogenous hydrocortisone can be distinguished.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hydrocortisone
/
Doping in Sports
/
Horses
/
Gas Chromatography-Mass Spectrometry
/
Anti-Inflammatory Agents
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Biomed Sci Appl
Journal subject:
QUIMICA CLINICA
Year:
1997
Document type:
Article
Affiliation country:
Francia