Visualization of F-actin filaments by a fluorescently labeled nucleotide analogue.
Biophys Chem
; 75(1): 1-6, 1998 Oct 05.
Article
in En
| MEDLINE
| ID: mdl-9810684
ABSTRACT
The tightly-bound nucleotide of F-actin was replaced with 1,N6-etheno-adenosine ATP (ADP). An epi-fluorescence optical microscope was modified to visualize efficiently the fluorescent analogue with an excitation-maximum wavelength of 310 nm. This microscope permitted us to visualize single F-actin filaments in solution using the fluorescence of the strongly bound 1,N6-ethenoadenosine nucleotide. Exchange of the tightly-bound nucleotide of F-actin with a free nucleotide in solution at a high temperature was quantitatively estimated by this method, and the results showed good agreement with results from phosphate release measurements.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Actins
Language:
En
Journal:
Biophys Chem
Year:
1998
Document type:
Article
Affiliation country:
Japón