Quantitative abnormalities of fetal DNA in maternal serum in preeclampsia.
Clin Chem
; 45(2): 184-8, 1999 Feb.
Article
in En
| MEDLINE
| ID: mdl-9931039
ABSTRACT
BACKGROUND:
There is much recent interest in the biologic and diagnostic implication of cell-free non-host DNA in the plasma and serum of human subjects. To determine if quantitative abnormalities of circulating non-host DNA may be associated with certain pathologic processes, we used circulating fetal DNA in preeclampsia as a model system.METHODS:
We studied 20 preeclamptic women and 20 control subjects of comparable gestational age (means, 32 and 33 weeks, respectively). Male fetal DNA in maternal serum was measured using real-time quantitative PCR for the SRY gene on the Y chromosome.RESULTS:
The imprecision (CV) of the assay was 2.7%. The median circulating fetal DNA was increased fivefold in 20 preeclamptic women compared with 20 control pregnant women (381 vs 76 genome-equivalents/mL, P <0.001).CONCLUSIONS:
These observations suggest that preeclampsia is associated with disturbances in the liberation and/or clearance mechanisms of circulating DNA. These results also raise the possibility that measurement of circulating DNA may prove useful as a marker for the diagnosis and/or monitoring of preeclampsia.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pre-Eclampsia
/
DNA
/
Fetus
/
Mothers
Type of study:
Observational_studies
/
Risk_factors_studies
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Language:
En
Journal:
Clin Chem
Journal subject:
QUIMICA CLINICA
Year:
1999
Document type:
Article