Protective effect of complement C5a receptor 1 antagonist on cerebral ischemia-reperfusion in mice / 中风与神经疾病杂志

ABSTRACT

@#Objective　To investigate the protective effect of complement C5a receptor 1 (C5aR1) antagonist on cerebral ischemia-reperfusion （CIR） in mice.Method　Mice were randomly divided into sham operation group,cerebral ischemia-reperfusion group (model group) and C5aR1 antagonist （PMX53）group.At 3 h before,24 h after and 48 h after cerebral reperfusion timepoint,the PMX53 group was given with PMX53,the sham group and the model group were given same volume of saline by intraperitoneall injection.At 72 h after cerebral reperfusion timepoint,neurological deficits score of mice were evaluated by the modified Longa method,the infarcted brain volume was calculated after TTC staining,the cerebral tissue water content of the ischemic hemisphere was calculated by dry and wet weight method,the mRNA expression of inflammatory factors in the ischemic hemisphere were detected by real-time PCR,and the relative expression of ZO-1 in cerebral tissue of the ischemic hemisphere was calculated by Western blot.Results　At 72 h after cerebral reperfusion,compared with the model group,neurological deficits function score,cerebral water content,cerebral infarction volume and inflammatory cytokines (IL-1β,TNF-α) were significantly decreased in the PMX53 group (all P<0.05),ZO-1 expression was significantly higher in the PMX53 group (P<0.05).Conclusion　C5aR1 antagonist can improve the neurological function score after CIR,reduce the volume of cerebral infarction,reduce the degree of cerebral edema and inflammatory response,and has a protective effect on the blood-brain barrier.