The Effect of Combined Immunosuppressive Treatment with FK 506-Cyclophosphamide on Surgical Angiogenesis / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
; : 58-64, 2006.
Article
in Ko
| WPRIM
| ID: wpr-143405
Responsible library:
WPRO
ABSTRACT
PURPOSE:
To evaluate the effect of combined immunosuppressive treatment with FK 506-cyclophosphamide on surgical angiogenesis in non-vascularized bone xenografts. MATERIALS ANDMETHODS:
Twenty-seven hamster-to-rat non-vascularized bone xenografts were performed. For surgical angiogenesis, recipient origin saphenous arteriovenous bundles were implanted into the medullary canals of graft bones. Immunosuppression with FK 506-cyclophosphamide was performed in group 1, not in group 2, control group. At postoperative 4 weeks, microangiography with microfil was conducted. Decalcified bones were cleared and the area of newly formed capillary, capillary density, were quantitatively evaluated using Scion image program. Bone specimens stained with hematoxylin-eosin were evaluated histologically also.RESULTS:
There was no significant difference of capillary densities between immunosuppressed group and non-immunosuppressed control group (0.37+/-0.03/0.39+/-0.02, P=0.58). On histological examination, both groups showed neoangiogenesis into the medullary canals and endosteal bones.CONCLUSION:
FK 506-cyclophosphamide immunosuppression did not suppress the angiogenesis in non-vascularized bone xenografts, FK 506-cyclophosphamide combination of immunosuppression will be useful even in the condition of surgical angiogenesis.Key words
Full text:
1
Database:
WPRIM
Main subject:
Silicone Elastomers
/
Capillaries
/
Immunosuppression Therapy
/
Transplants
/
Heterografts
Language:
Ko
Journal:
Journal of Korean Orthopaedic Research Society
Year:
2006
Document type:
Article